Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza
Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza (EC 2.5.1.98, PssM) je enzim sa sistematskim imenom fosfoenolpiruvat:(D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-(3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6))-2(or3)-O-Ac-D-Glc-alfa-(1->6))n 4,6-O-(1-karboksiethan-1,1-diil)transferaza.[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.98 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- fosfoenolpiruvat + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(ili 3)-O-Ac-D-Glc-alfa-(1->6)]n [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal--beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(ili 3)-O-Ac-D-Glc-alfa-(1->6)]n + fosfat
Ovaj enzim je odgovoran za piruvilaciju subterminalne glukoze u kiseloj oktasaharidnoj ponavljajućoj jedinici eksopolisaharida kod Rhizobium leguminosarum (bv. viciae soj VF39), koja je neophodna za uspostavlanje azot-fiksirajuće simbioze sa Pisum sativum, Vicia faba, i Vicia sativa.
Reference
уреди- ^ Ivashina, T.V., Fedorova, E.E., Ashina, N.P., Kalinchuk, N.A., Druzhinina, T.N., Shashkov, A.S., Shibaev, V.N. and Ksenzenko, V.N. (2010). „Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae—Pisum sativum symbiosis”. J. Appl. Microbiol. 109: 731—742. PMID 20233262.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- Rhizobium+leguminosarum+exopolysaccharide+glucosyl+ketal-pyruvate-transferase на US National Library of Medicine Medical Subject Headings (MeSH)
