2-Alkenal reduktaza
2-Alkenal reduktaza (EC 1.3.1.74, NAD(P)H-zavisni alkenal/on oksidoreduktaza, NADPH:2-alkenal alfa,beta-hidrogenaza) je enzim sa sistematskim imenom n-alkanal:NAD(P)+ 2-oksidoreduktaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Alkenal reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Leukotriene B4 12-hydroxydehydrogenase (NADP-dependent) dimer, Human | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.3.1.74 | ||||||||
| CAS broj | 52227-95-9 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- N-alkanal + NAD(P)+ alk-2-enal + NAD(P)H + H+
Ovaj enzim je visoko specifičan za 4-hidroksinon-2-enal i non-2-enal. 2-Alkenali kraćih lanaca imaju niže afinitete. Ovaj enzim je veoma aktivan na 2-alkenonima, kao što su but-3-en-2-on i pent-3-en-2-on. On je neaktivan na cikloheks-2-en-1-onu i 12-oksofitodienoinskoj kiselini. On učestvuje u detoksikaciji alfa,beta-nezasićenih aldehida i ketona.
Reference uredi
- ^ Mano, J., Torii, Y., Hayashi, S., Takimoto, K., Matsui, K., Nakamura, K., Inzé, D., Babiychuk, E., Kushnir, S. and Asada, K. (2002). „The NADPH:quinone oxidoreductase P1-ζ-crystallin in Arabidopsis catalyzes the α,β-hydrogenation of 2-alkenals: detoxication of the lipid peroxide-derived reactive aldehydes”. Plant Cell Physiol. 43: 1445—1455. PMID 12514241.
- ^ Dick, R.A., Kwak, M.K., Sutter, T.R. and Kensler, T.W. (2001). „Antioxidative function and substrate specificity of NAD(P)H-dependent alkenal/one oxidoreductase. A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase”. J. Biol. Chem. 276: 40803—40810. PMID 11524419.
Literatura uredi
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze uredi
- 2-alkenal+reductase на US National Library of Medicine Medical Subject Headings (MeSH)
