Restrikcioni enzim — разлика између измена
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Ред 1:
'''Restrikcioni enzim''' (''restrikciona [[endonukleaza]]'') je [[enzim]] koji preseca [[DNK]] na specifičnim prepoznatljivim [[nukleotid]]nim sekvencama poznatim kao [[restrikciono mesto|restrikciona mesta]]. Tip II restrikcioni enzimi presecaju dvolančanu [[DNK]].<ref name="pmid795607">{{cite journal | author = Roberts RJ | title = Restriction endonucleases | journal = CRC Crit. Rev. Biochem. | volume = 4 | issue = 2 | pages = 123–64 | year = 1976 | month = November | pmid = 795607 | doi = 10.3109/10409237609105456| url = | last2 = Murray | first2 = Kenneth }}</ref><ref name="pmid2172084">{{cite journal | author = Kessler C, Manta V | title = Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3) | journal = Gene | volume = 92 | issue = 1–2 | pages = 1–248 | year = 1990 | month = August | pmid = 2172084 | doi = 10.1016/0378-1119(90)90486-B | url = }}</ref><ref name="isbn0-89603-234-5">{{Cite book | author = Pingoud A, Alves J, Geiger R | authorlink = | editor = Burrell, Michael | others = | title = Enzymes of Molecular Biology | edition = | language = | publisher = Humana Press | location = Totowa, NJ | series= Methods of Molecular Biology | volume= 16 | year = 1993 | origyear = | pages = 107–200 | chapter = Chapter 8: Restriction Enzymes | quote = | isbn = 0-89603-234-5 | oclc = | doi = | url = | accessdate = }}</ref> Ovi enzimi, nađeni kod [[bakterija]] i [[arheja]], su verovatno nastali kao odbrambeni mehanizam protiv [[virus]]ne invazije.<ref name="pmid4897066">{{cite journal | author = Arber W, Linn S | title = DNA modification and restriction | journal = Annu. Rev. Biochem. | volume = 38 | issue = | pages = 467–500 | year = 1969 | pmid = 4897066 | doi = 10.1146/annurev.bi.38.070169.002343 | url = }}</ref><ref name="pmid6314109">{{cite journal | author = Krüger DH, Bickle TA | title = Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts | journal = Microbiol. Rev. | volume = 47 | issue = 3 | pages = 345–60 | year = 1983 | month = September | pmid = 6314109 | pmc = 281580 | doi = | url = }}</ref> Unutar bakterije, restricioni enzimi selektivno presecaju ''stranu'' DNK u procesu ''restrikcije''. DNK domaćina je [[Metilacija|metilisana]] putem modifikacionog enzima ([[Metiltransferaza|metilaze]]) da bi se zaštitila od dejstva restrikcionih enzima. Kolektivno, ta dva procesa formiraju [[restrikcioni modifikacioni sistem]].<ref name="pmid11557807">{{cite journal | author = Kobayashi I | title = Behavior of restriction–modification systems as selfish mobile elements and their impact on genome evolution | journal = Nucleic Acids Res. | volume = 29 | issue = 18 | pages = 3742–56 | year = 2001 | month = September | pmid = 11557807 | pmc = 55917 | doi = 10.1093/nar/29.18.3742 | url = | accessdate = }}</ref> Da bi presekao DNK, restrikcioni enzim pravi dva zaseka, jednom kroz svaki šećerno-fosfatni lanac DNK dvostrukog heliksa.
Preko 3000 restrikcionih enzima je detaljno ispitano, i više od 600 je komercijalno dostupno<ref name="pmid17202163">{{cite journal | journal=Nucleic Acids Res | volume=35 | issue=Database issue | pages=D269–70 | year=2007 | author=Roberts RJ, Vincze T, Posfai J, Macelis D. | title=REBASE—enzymes and genes for DNA restriction and modification | pmid=17202163 | doi = 10.1093/nar/gkl891 | pmc=1899104 }}</ref> i rutinski se koristi za DNK modifikaciju i manipulaciu u laboratorijama.<ref name="isbn0-632-03712-1">{{Cite book | author = Primrose, Sandy B.; Old, R. W. | authorlink = | editor = | others = | title = Principles of gene manipulation: an introduction to genetic engineering | edition = | language = | publisher = Blackwell Scientific | location = Oxford | year = 1994 | origyear = | pages = | quote = | isbn = 0-632-03712-1 | oclc = | doi = | url = | accessdate = }}</ref><ref name="isbn0-8053-3040-2">{{Cite book | author = Micklos, David A.; Bloom, Mark V.; Freyer, Greg A. | authorlink = | editor = | others = | title = Laboratory DNA science: an introduction to recombinant DNA techniques and methods of genome analysis | edition = | language = | publisher = Benjamin/Cummings Pub. Co | location = Menlo Park, Calif | year = 1996 | origyear = | pages = | quote = | isbn = 0-8053-3040-2 | oclc = | doi = | url = | accessdate = }}</ref><ref name="isbn1-55581-176-0">{{
== Reference ==
Ред 12:
* {{cite web | url = http://www.scq.ubc.ca/?p=249 | title = Restriction Endonucleases: Molecular Scissors for Specifically Cutting DNA | author = Simmer M, Secko D | authorlink = | coauthors = | date = 01. 08. 2003. | format = | work = The Science Creative Quarterly | publisher = | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = 06. 06. 2008.}}
* {{Cite book | author = Pingoud A, Alves J, Geiger R | authorlink = | editor = Burrell, Michael | others = | title = Enzymes of Molecular Biology | edition = | language = | publisher = Humana Press | location = Totowa, NJ | series= Methods of Molecular Biology | volume= 16 | year = 1993 | origyear = | pages = 107–200 | chapter = Chapter 8: Restriction Enzymes | quote = | isbn = 0-89603-234-5 | oclc = | doi = | url = | accessdate = | ref = harv}}
* {{Cite book | author = Primrose, Sandy B.; Old, R. W. | authorlink = | editor = | others = | title = Principles of gene manipulation: an introduction to genetic engineering | edition = | language = | publisher = Blackwell Scientific | location = Oxford | year = 1994 | origyear = | pages = | quote = | isbn = 0-632-03712-1 | oclc = | doi = | url = | accessdate = | ref = harv}}
* {{Cite book | author = Micklos, David A.; Bloom, Mark V.; Freyer, Greg A. | authorlink = | editor = | others = | title = Laboratory DNA science: an introduction to recombinant DNA techniques and methods of genome analysis | edition = | language = | publisher = Benjamin/Cummings Pub. Co | location = Menlo Park, Calif | year = 1996 | origyear = | pages = | quote = | isbn = 0-8053-3040-2 | oclc = | doi = | url = | accessdate = }}
* {{Cite book | author = Adrianne Massey; Helen Kreuzer | authorlink = | editor = | others = | title = Recombinant DNA and Biotechnology: A Guide for Students | edition = | language = | publisher = ASM Press | location = Washington, D.C | year = 2001 | origyear = | pages = | quote = | isbn = 1-55581-176-0 | oclc = | doi = | url = | accessdate = }}
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