Restrikcioni enzim — разлика између измена
Садржај обрисан Садржај додат
м референце |
м ispravke |
||
Ред 1:
'''Restrikcioni enzim''' (''restrikciona [[endonukleaza]]'') je [[enzim]] koji preseca [[DNK]] na specifičnim prepoznatljivim [[nukleotid]]nim sekvencama poznatim kao [[restrikciono mesto|restrikciona mesta]]. Tip II restrikcioni enzimi presecaju dvolančanu [[DNK]].<ref name="pmid795607">{{cite journal | author = Roberts RJ | title = Restriction endonucleases | journal = CRC Crit. Rev. Biochem. | volume = 4 | issue = 2 | pages=123-64 | year = 1976 | month = November | pmid = 795607 | doi = 10.3109/10409237609105456| url = | last2 = Murray | first2 = Kenneth }}</ref><ref name="pmid2172084">{{cite journal | author = Kessler C, Manta V | title = Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3) | journal = Gene | volume = 92 | issue = 1–2 | pages=1-248 | year = 1990 | month = August | pmid = 2172084 | doi = 10.1016/0378-1119(90)90486-B | url = }}</ref><ref name="isbn0-89603-234-5">{{Cite book |author=Pingoud A, Alves J, Geiger R | authorlink = | editor = Burrell, Michael | others = | title = Enzymes of Molecular Biology | edition = | language = | publisher = Humana Press | location = Totowa, NJ | series= Methods of Molecular Biology | volume= 16 |year=1993 | origyear = |pages=107-200 | chapter = Chapter 8: Restriction Enzymes | quote = |id=ISBN 0-89603-234-5 | oclc = | doi = |url=| accessdate = }}</ref> Ovi enzimi, nađeni kod [[bakterija]] i [[arheja]], su verovatno nastali kao odbrambeni mehanizam protiv [[virus]]ne invazije.<ref name="pmid4897066">{{cite journal |
Preko 3000 restrikcionih enzima je detaljno ispitano, i više od 600 je komercijalno dostupno<ref name="pmid17202163">{{cite journal | journal=Nucleic Acids Res | volume=35 | issue=Database issue | pages=D269–70 | year=2007 | author=Roberts RJ, Vincze T, Posfai J, Macelis D. | title=REBASE—enzymes and genes for DNA restriction and modification | pmid=17202163 | doi = 10.1093/nar/gkl891 | pmc=1899104 }}</ref> i rutinski se koristi za DNK modifikaciju i manipulaciu u laboratorijama.<ref name="isbn0-632-03712-1">{{Cite book |author=Primrose, Sandy B.; Old, R. W. | authorlink = | editor = | others = | title = Principles of gene manipulation: an introduction to genetic engineering | edition = | language = | publisher = Blackwell Scientific | location = Oxford |year=1994 | origyear = |pages=| quote = |id=ISBN 0-632-03712-1 | oclc = | doi = |url=| accessdate = }}</ref><ref name="isbn0-8053-3040-2">{{Cite book |author=Micklos, David A.; Bloom, Mark V.; Freyer, Greg A. | authorlink = | editor = | others = | title = Laboratory DNA science: an introduction to recombinant DNA techniques and methods of genome analysis | edition = | language = | publisher = Benjamin/Cummings Pub. Co | location = Menlo Park, Calif |year=1996 | origyear = |pages=| quote = |id=ISBN 0-8053-3040-2 | oclc = | doi = |url=| accessdate = }}</ref><ref name="isbn1-55581-176-0">{{Cite book |author=Adrianne Massey; Helen Kreuzer | authorlink = | editor = | others = | title = Recombinant DNA and Biotechnology: A Guide for Students | edition = | language = | publisher = ASM Press | location = Washington, D.C |year=2001 | origyear = |pages=| quote = |id=ISBN 1-55581-176-0 | oclc = | doi = |url=| accessdate = }}</ref>
|