Germakren-A sintaza
(преусмерено са (+)-germakren A sintaza)
Germakren-A sintaza (EC 4.2.3.23, germakren A sintaza, (+)-germakren A sintaza, (+)-(10R)-germakren A sintaza, GAS, 2-trans,6-trans-farnezil-difosfat difosfat-lijaza (formira germakren-A)) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat difosfat-lijaza (formira (+)-(R)-germakren-A).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Germakren-A sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.3.23 | ||||||||
| CAS broj | 213763-55-4 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (2E,6E)-farnezil difosfat (+)-(R)-gemakren A + difosfat
Za dejstvo ovog enzima je neophodan jon Mg2+.
Reference уреди
- ^ Bouwmeester, H.J., Kodde, J., Verstappen, F.W., Altug, I.G., de Kraker, J.W. and Wallaart, T.E. (2002). „Isolation and characterization of two germacrene A synthase cDNA clones from chicory”. Plant Physiol. 129 (1): 134—144. PMC 155878
. PMID 12011345. doi:10.1104/pp.001024.
- ^ Prosser, I., Phillips, A.L., Gittings, S., Lewis, M.J., Hooper, A.M., Pickett, J.A. and Beale, M.H. (2002). „(+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis”. Phytochemistry. 60 (7): 691—702. PMID 12127586. doi:10.1016/s0031-9422(02)00165-6.
- ^ de Kraker, J.W., Franssen, M.C., de Groot, A., König, W.A. and Bouwmeester, H.J. (1998). „(+)-Germacrene A biosynthesis . The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory”. Plant Physiol. 117 (4): 1381—1392. PMC 34902 . PMID 9701594. doi:10.1104/pp.117.4.1381.
- ^ Calvert, M.J., Ashton, P.R. and Allemann, R.K. (2002). „Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene”. J. Am. Chem. Soc. 124 (39): 11636—11641. PMID 12296728. doi:10.1021/ja020762p.
- ^ Chang, Y.J., Jin, J., Nam, H.Y. and Kim, S.U. (2005). „Point mutation of (+)-germacrene A synthase from Ixeris dentata”. Biotechnol. Lett. 27 (5): 285—288. PMID 15834787. S2CID 21959582. doi:10.1007/s10529-005-0681-9.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze уреди
- Germacrene-A+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
