Replikacioni faktor C, podjedinica 5 je protein koji je kod ljudi kodiran RFC5 genom[1][2].

Replikacioni faktor C (aktivator 1) 5, 36.5kDa
Dostupne strukture
1LFS
Identifikatori
Simboli RFC5; RFC36
Vanjski ID OMIM600407 HomoloGene6730 GeneCards: RFC5 Gene
Pregled RNK izražavanja
podaci
Ortolozi
Vrsta Čovek Miš
Entrez 5985 72151
Ensembl ENSG00000111445 ENSMUSG00000029363
UniProt P40937 Q9D0F6
Ref. Sekv. (iRNK) NM_001130112 NM_028128
Ref. Sekv. (protein) NP_001123584 NP_082404
Lokacija (UCSC) Chr 12:
118.45 - 118.47 Mb
Chr 5:
117.38 - 117.39 Mb
PubMed pretraga [1] [2]

Za elongaciju prajmiranog DNK šablona DNK polimeraze delta i DNK polimeraze epsilon neophodni su pomoćni proteini: proliferativni ćelijski nuklearni antigen (PCNA) i replikacioni faktor C (RFC). RFC, takođe poznat kao aktivator 1, je proteinski kompleks koji se sastoji od pet podjedinica sa 140, 40, 38, 37, i 36 kDa. Ovaj gen kodira 36 kDa podjednicu. Ta podjedinica interaguje sa C-terminalnim regionom PCNA. Ona formira sržni kompleks sa 38 i 40 kDa podjedinicama. Sržni kompleks ispoljava ATPaznu aktivnost, koju stimuliše PCNA u in vitro sistemima. Poznate su alternativno splajsovane transcriptne varijante koje kodiraju različite izoforme.[2]

Interakcije

uredi

RFC5 formira interakcije sa BRD4,[3] RFC2,[4][5] PCNA,[4][6][7] RFC4[4][5][8][9] i CHTF18.[10]

Reference

uredi
  1. ^ Okumura K; Nogami M; Taguchi H; Dean FB; Chen M; Pan ZQ; Hurwitz J; Shiratori A; Murakami Y; Ozawa K; et al. (jul 1995). „Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23”. Genomics. 25 (1): 274—8. PMID 7774928. doi:10.1016/0888-7543(95)80135-9. 
  2. ^ a b „Entrez Gene: RFC5 replication factor C (activator 1) 5, 36.5kDa”. 
  3. ^ Maruyama, Tetsuo; Farina Andrea; et al. (2002). „A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase”. Mol. Cell. Biol. United States. 22 (18): 6509—20. ISSN 0270-7306. PMC 135621 . PMID 12192049. doi:10.1128/MCB.22.18.6509-6520.2002. 
  4. ^ а б в Cai, J; Gibbs E; et al. (1997). „A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme”. J. Biol. Chem. UNITED STATES. 272 (30): 18974—81. ISSN 0021-9258. PMID 9228079. doi:10.1074/jbc.272.30.18974. 
  5. ^ а б Cai, J; Yao N; et al. (1998). „ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits”. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 95 (20): 11607—12. ISSN 0027-8424. PMC 21688 . PMID 9751713. doi:10.1073/pnas.95.20.11607. 
  6. ^ Mossi, R; Jónsson Z O; et al. (1997). „Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen”. J. Biol. Chem. UNITED STATES. 272 (3): 1769—76. ISSN 0021-9258. PMID 8999859. doi:10.1074/jbc.272.3.1769. 
  7. ^ Ohta, Satoshi; Shiomi Yasushi; et al. (2002). „A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein”. J. Biol. Chem. United States. 277 (43): 40362—7. ISSN 0021-9258. PMID 12171929. doi:10.1074/jbc.M206194200. 
  8. ^ Rual, Jean-François; Venkatesan Kavitha; et al. (2005). „Towards a proteome-scale map of the human protein-protein interaction network”. Nature. England. 437 (7062): 1173—8. PMID 16189514. doi:10.1038/nature04209. 
  9. ^ Ellison, V; Stillman B (1998). „Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity”. J. Biol. Chem. UNITED STATES. 273 (10): 5979—87. ISSN 0021-9258. PMID 9488738. doi:10.1074/jbc.273.10.5979. 
  10. ^ Bermudez, Vladimir P; Maniwa Yoshimasa; et al. (2003). „The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA”. Proc. Natl. Acad. Sci. U.S.A. United States. 100 (18): 10237—42. ISSN 0027-8424. PMC 193545 . PMID 12930902. doi:10.1073/pnas.1434308100. 

Литература

uredi