Glutamin-(asparagin-)aza (EC 3.5.1.38, glutaminaza-asparaginaza, ansB (gen), L-asparagin/L-glutamin amidohidrolaza, L-ASNaza/L-GLNaza) je enzim sa sistematskim imenom L-glutamin(L-asparagin) amidohidrolaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

Glutamin-(asparagin-)aza
Identifikatori
EC broj3.5.1.38
CAS broj39335-03-0
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum
(1) L-glutamin + H2O L-glutamat + NH3
(2) L-asparagin + H2O L-aspartat + NH3

Enzim iz bakterije Achromobacter hidrolizuje L-asparagin sa 0.8 brzine hidrolize L-glutamina.

Reference

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  1. ^ Roberts, J., Holcenberg, J.S. and Dolowy, W.C. (1972). „Isolation, crystallization, and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity”. J. Biol. Chem. 247: 84—90. PMID 5017769. 
  2. ^ Tanaka, S., Robinson, E.A., Appella, E., Miller, M., Ammon, H.L., Roberts, J., Weber, I.T. and Wlodawer, A. (1988). „Structures of amidohydrolases. Amino acid sequence of a glutaminase-asparaginase from Acinetobacter glutaminasificans and preliminary crystallographic data for an asparaginase from Erwinia chrysanthemi”. J. Biol. Chem. 263: 8583—8591. PMID 3379033. 
  3. ^ Lubkowski, J., Wlodawer, A., Ammon, H.L., Copeland, T.D. and Swain, A.L. (1994). „Structural characterization of Pseudomonas 7A glutaminase-asparaginase”. Biochemistry. 33: 10257—10265. PMID 8068664. 
  4. ^ Ortlund, E., Lacount, M.W., Lewinski, K. and Lebioda, L. (2000). „Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu”. Biochemistry. 39: 1199—1204. PMID 10684596. 

Literatura

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Spoljašnje veze

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