RFC5
Репликациони фактор Ц, подјединица 5 је протеин који је код људи кодиран RFC5 геном[1][2].
| Replikacioni faktor C (aktivator 1) 5, 36.5kDa | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Доступне структуре | |||||||||||
| 1LFS | |||||||||||
| Идентификатори | |||||||||||
| Симболи | RFC5; RFC36 | ||||||||||
| Вањски ИД | OMIM: 600407 HomoloGene: 6730 GeneCards: RFC5 Gene | ||||||||||
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| Преглед РНК изражавања | |||||||||||
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| подаци | |||||||||||
| Ортолози | |||||||||||
| Врста | Човек | Миш | |||||||||
| Ентрез | 5985 | 72151 | |||||||||
| Eнсембл | ENSG00000111445 | ENSMUSG00000029363 | |||||||||
| UниПрот | P40937 | Q9D0F6 | |||||||||
| Реф. Секв. (иРНК) | NM_001130112 | NM_028128 | |||||||||
| Реф. Секв. (протеин) | NP_001123584 | NP_082404 | |||||||||
| Локација (УЦСЦ) |
Chr 12: 118.45 - 118.47 Mb |
Chr 5: 117.38 - 117.39 Mb | |||||||||
| ПубМед претрага | [1] | [2] | |||||||||
За елонгацију прајмираног ДНК шаблона ДНК полимеразе делта и ДНК полимеразе епсилон неопходни су помоћни протеини: пролиферативни ћелијски нуклеарни антиген (ПЦНА) и репликациони фактор Ц (RFC). RFC, такође познат као активатор 1, је протеински комплекс који се састоји од пет подјединица са 140, 40, 38, 37, и 36 kDа. Овај ген кодира 36 kDа подједницу. Та подјединица интерагује са Ц-терминалним регионом ПЦНА. Она формира сржни комплекс са 38 и 40 kDа подјединицама. Сржни комплекс испољава АТПазну активност, коју стимулише ПЦНА у ин витро системима. Познате су алтернативно сплајсоване трансцриптне варијанте које кодирају различите изоформе.[2]
Интеракције
уредиRFC5 формира интеракције са BRD4,[3] RFC2,[4][5] PCNA,[4][6][7] RFC4[4][5][8][9] i CHTF18.[10]
Референце
уреди- ^ Okumura K; Nogami M; Taguchi H; Dean FB; Chen M; Pan ZQ; Hurwitz J; Shiratori A; Murakami Y; Ozawa K; et al. (јул 1995). „Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23”. Genomics. 25 (1): 274—8. PMID 7774928. doi:10.1016/0888-7543(95)80135-9.
- ^ а б „Entrez Gene: RFC5 replication factor C (activator 1) 5, 36.5kDa”.
- ^ Maruyama, Tetsuo; Farina Andrea; et al. (2002). „A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase”. Mol. Cell. Biol. United States. 22 (18): 6509—20. ISSN 0270-7306. PMC 135621
. PMID 12192049. doi:10.1128/MCB.22.18.6509-6520.2002.
- ^ а б в Cai, J; Gibbs E; et al. (1997). „A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme”. J. Biol. Chem. UNITED STATES. 272 (30): 18974—81. ISSN 0021-9258. PMID 9228079. doi:10.1074/jbc.272.30.18974.
- ^ а б Cai, J; Yao N; et al. (1998). „ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits”. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 95 (20): 11607—12. ISSN 0027-8424. PMC 21688 . PMID 9751713. doi:10.1073/pnas.95.20.11607.
- ^ Mossi, R; Jónsson Z O; et al. (1997). „Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen”. J. Biol. Chem. UNITED STATES. 272 (3): 1769—76. ISSN 0021-9258. PMID 8999859. doi:10.1074/jbc.272.3.1769.
- ^ Ohta, Satoshi; Shiomi Yasushi; et al. (2002). „A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein”. J. Biol. Chem. United States. 277 (43): 40362—7. ISSN 0021-9258. PMID 12171929. doi:10.1074/jbc.M206194200.
- ^ Rual, Jean-François; Venkatesan Kavitha; et al. (2005). „Towards a proteome-scale map of the human protein-protein interaction network”. Nature. England. 437 (7062): 1173—8. PMID 16189514. doi:10.1038/nature04209.
- ^ Ellison, V; Stillman B (1998). „Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity”. J. Biol. Chem. UNITED STATES. 273 (10): 5979—87. ISSN 0021-9258. PMID 9488738. doi:10.1074/jbc.273.10.5979.
- ^ Bermudez, Vladimir P; Maniwa Yoshimasa; et al. (2003). „The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA”. Proc. Natl. Acad. Sci. U.S.A. United States. 100 (18): 10237—42. ISSN 0027-8424. PMC 193545 . PMID 12930902. doi:10.1073/pnas.1434308100.
Литература
уреди- O'Donnell M; Onrust R; Dean FB; et al. (1993). „Homology in accessory proteins of replicative polymerases--E. coli to humans.”. Nucleic Acids Res. 21 (1): 1—3. PMC 309057 . PMID 8441605. doi:10.1093/nar/21.1.1.
- Uhlmann F; Cai J; Flores-Rozas H; et al. (1996). „In vitro reconstitution of human replication factor C from its five subunits.”. Proc. Natl. Acad. Sci. U.S.A. 93 (13): 6521—6. PMC 39056 . PMID 8692848. doi:10.1073/pnas.93.13.6521.
- Mossi R; Jónsson ZO; Allen BL; et al. (1997). „Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen.”. J. Biol. Chem. 272 (3): 1769—76. PMID 8999859. doi:10.1074/jbc.272.3.1769.
- Cujec TP; Cho H; Maldonado E; et al. (1997). „The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.”. Mol. Cell. Biol. 17 (4): 1817—23. PMC 232028 . PMID 9121429.
- Cai J; Gibbs E; Uhlmann F; et al. (1997). „A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme.”. J. Biol. Chem. 272 (30): 18974—81. PMID 9228079. doi:10.1074/jbc.272.30.18974.
- Loor G; Zhang SJ; Zhang P; et al. (1998). „Identification of DNA replication and cell cycle proteins that interact with PCNA.”. Nucleic Acids Res. 25 (24): 5041—6. PMC 147130 . PMID 9396813. doi:10.1093/nar/25.24.5041.
- Ellison V, Stillman B (1998). „Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity.”. J. Biol. Chem. 273 (10): 5979—87. PMID 9488738. doi:10.1074/jbc.273.10.5979.
- Cai J; Yao N; Gibbs E; et al. (1998). „ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits.”. Proc. Natl. Acad. Sci. U.S.A. 95 (20): 11607—12. PMC 21688 . PMID 9751713. doi:10.1073/pnas.95.20.11607.
- Zhang G; Gibbs E; Kelman Z; et al. (1999). „Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen.”. Proc. Natl. Acad. Sci. U.S.A. 96 (5): 1869—74. PMC 26703 . PMID 10051561. doi:10.1073/pnas.96.5.1869.
- Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (2001). „Purification and characterization of human DNA damage checkpoint Rad complexes.”. Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11236—41. PMC 58713 . PMID 11572977. doi:10.1073/pnas.201373498.
- Griffith JD, Lindsey-Boltz LA, Sancar A (2002). „Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy.”. J. Biol. Chem. 277 (18): 15233—6. PMID 11907025. doi:10.1074/jbc.C200129200.
- Ohta S; Shiomi Y; Sugimoto K; et al. (2002). „A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein.”. J. Biol. Chem. 277 (43): 40362—7. PMID 12171929. doi:10.1074/jbc.M206194200.
- Maruyama T; Farina A; Dey A; et al. (2002). „A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase.”. Mol. Cell. Biol. 22 (18): 6509—20. PMC 135621 . PMID 12192049. doi:10.1128/MCB.22.18.6509-6520.2002.
- Venclovas C, Colvin ME, Thelen MP (2003). „Molecular modeling-based analysis of interactions in the RFC-dependent clamp-loading process.”. Protein Sci. 11 (10): 2403—16. PMC 2373712 . PMID 12237462. doi:10.1110/ps.0214302.
- Strausberg RL; Feingold EA; Grouse LH; et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899—903. PMC 139241 . PMID 12477932. doi:10.1073/pnas.242603899.
- Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (2003). „Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment.”. J. Biol. Chem. 278 (32): 30051—6. PMID 12766176. doi:10.1074/jbc.M211591200.
- Bermudez VP; Maniwa Y; Tappin I; et al. (2003). „The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.”. Proc. Natl. Acad. Sci. U.S.A. 100 (18): 10237—42. PMC 193545 . PMID 12930902. doi:10.1073/pnas.1434308100.
- Zou L, Liu D, Elledge SJ (2004). „Replication protein A-mediated recruitment and activation of Rad17 complexes.”. Proc. Natl. Acad. Sci. U.S.A. 100 (24): 13827—32. PMC 283506 . PMID 14605214. doi:10.1073/pnas.2336100100.
- Ota T; Suzuki Y; Nishikawa T; et al. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs.”. Nat. Genet. 36 (1): 40—5. PMID 14702039. doi:10.1038/ng1285.