(S)-2-hidroksipropilfosfonska kiselina epoksidaza
(S)-2-hidroksipropilfosfonska kiselina epoksidaza (EC 1.14.19.7, HPP epoksidaza, HppE, 2-hidroksipropilfosfonic kiselina epoksidaza, Fom4, (S)-2-hidroksipropilfosfonat epoksidaza) je enzim sa sistematskim imenom (S)-2-hidroksipropilfosfonat,NADH:kiseonik epoksidaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| (S)-2-hidroksipropilfosfonska kiselina epoksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.19.7 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (S)-2-hidroksipropilfosfonat + NADH + H+ + O2 (1R,2S)-epoksipropilfosfonat + NAD+ + 2H2O
Ovaj enzim sadrži u svakom monomeru jedan center gvožđa koje nije vezano za hem.
Reference
уреди- ^ Munos, J.W., Moon, S.J., Mansoorabadi, S.O., Chang, W., Hong, L., Yan, F., Liu, A. and Liu, H.W. (2008). „Purification and characterization of the epoxidase catalyzing the formation of fosfomycin from Pseudomonas syringae”. Biochemistry. 47: 8726—8735. PMID 18656958.
- ^ Yan, F., Moon, S.J., Liu, P., Zhao, Z., Lipscomb, J.D., Liu, A. and Liu, H.W. (2007). „Determination of the substrate binding mode to the active site iron of (S)-2-hydroxypropylphosphonic acid epoxidase using 17O-enriched substrates and substrate analogues”. Biochemistry. 46: 12628—12638. PMID 17927218.
- ^ Hidaka, T., Goda, M., Kuzuyama, T., Takei, N., Hidaka, M. and Seto, H. (1995). „Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis”. Mol. Gen. Genet. 249: 274—280. PMID 7500951.
- ^ Liu, P., Mehn, M.P., Yan, F., Zhao, Z., Que, L., Jr. and Liu, H.W. (2004). „Oxygenase activity in the self-hydroxylation of (S)-2-hydroxypropylphosphonic acid epoxidase involved in fosfomycin biosynthesis”. J. Am. Chem. Soc. 126: 10306—10312. PMID 15315444.
- ^ Higgins, L.J., Yan, F., Liu, P., Liu, H.W. and Drennan, C.L. (2005). „Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme”. Nature. 437: 838—844. PMID 16015285.
- ^ Cameron, S., McLuskey, K., Chamberlayne, R., Hallyburton, I. and Hunter, W.N. (2005). „Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61: 534—536. PMID 16511089.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- (S)-2-hydroxypropylphosphonic+acid+epoxidase на US National Library of Medicine Medical Subject Headings (MeSH)
