3-ketosteroid 9alfa-monooksigenaza
3-ketosteroid 9alfa-monooksigenaza (EC 1.14.13.142, KshAB, 3-ketosteroid 9alfa-hidroksilaza) je enzim sa sistematskim imenom androsta-1,4-dien-3,17-dion,NADH:kiseonik oksidoreduktaza (9alfa-hidroksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 3-ketosteroid 9alfa-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.142 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- androsta-1,4-dien-3,17-dion + NADH + H+ + O2 9alfa-hidroksiandrosta-1,4-dien-3,17-dion + NAD+ + H2O
Ovaj enzim učestvuje u holesterol degradaciji u nekoliko bakterijskih patogena, kao što je Mycobakterija tuberculosis.
Reference
уреди- ^ Petrusma, M., Dijkhuizen, L. and van der Geize, R. (2009). „Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9α-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity”. Appl. Environ. Microbiol. 75: 5300—5307. PMID 19561185.
- ^ Capyk, J.K., D'Angelo, I., Strynadka, N.C. and Eltis, L.D. (2009). „Characterization of 3-ketosteroid 9α-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis”. J. Biol. Chem. 284: 9937—9946. PMID 19234303.
- ^ Capyk, J.K., Casabon, I., Gruninger, R., Strynadka, N.C. and Eltis, L.D. (2011). „Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis”. J. Biol. Chem.: —. PMID 21987574.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- 3-ketosteroid+9alpha-monooxygenase на US National Library of Medicine Medical Subject Headings (MeSH)
