6,7-Dimetil-8-ribitillumazin sintaza
(преусмерено са 6,7-dimethyl-8-ribityllumazine synthase)
6,7-dimetil-8-ribitillumazin sintaza (EC 2.5.1.78, lumazinska sintaza, 6,7-dimetil-8-ribitillumazinska sintaza 2, 6,7-dimetil-8-ribitillumazinska sintaza 1, lumazinska sintaza 2, lumazinska sintaza 1, tip I lumazinska sintaza, tip II lumazinska sintaza, RIB4, MJ0303, RibH, Pbls, MbtLS, RibH1 protein, RibH2 protein, RibH1, RibH2) je enzim sa sistematskim imenom 5-amino-6-(D-ribitilamino)uracil butandiontransferaza.[1][2][3][4][5][6][7][8][9][10][11][12][13] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 6,7-dimetil-8-ribitillumazin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.78 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 1-dezoksi-L-glicero-tetruloza 4-fosfat + 5-amino-6-(D-ribitilamino)uracil 6,7-dimetil-8-(D-ribitil)lumazin + 2H2O + fosfat
Ovaj enzim učestvuje u biosintezi riboflavina.
Reference уреди
- ^ Kis, K., Volk, R. and Bacher, A. (1995). „Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase”. Biochemistry. 34: 2883—2892. PMID 7893702.
- ^ Garcia-Ramirez, J.J., Santos, M.A. and Revuelta, J.L. (1995). „The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein”. J. Biol. Chem. 270: 23801—23807. PMID 7559556.
- ^ Bacher, A., Fischer, M., Kis, K., Kugelbrey, K., Mörtl, S., Scheuring, J., Weinkauf, S., Eberhardt, S., Schmidt-Bäse, K., Huber, R., Ritsert, K., Cushman, M., Ladenstein, R. (1996). „Biosynthesis of riboflavin: structure and mechanism of lumazine synthase”. Biochem. Soc. Trans. 24: 89—94. PMID 8674771.
- ^ Mörtl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S. and Bacher, A. (1996). „Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli”. J. Biol. Chem. 271: 33201—33207. PMID 8969176.
- ^ Bacher, A., Eberhardt, S., Fischer, M., Mortl, S., Kis, K., Kugelbrey, K., Scheuring, J. and Schott, K. (1997). „Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase”. Methods Enzymol. 280: 389—399. PMID 9211334.
- ^ Goldbaum, F.A., Velikovsky, C.A., Baldi, P.C., Mortl, S., Bacher, A. and Fossati, C.A. (1999). „The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase”. J. Med. Microbiol. 48: 833—839. PMID 10482294.
- ^ Jordan, D.B., Bacot, K.O., Carlson, T.J., Kessel, M. and Viitanen, P.V. (1999). „Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase”. J. Biol. Chem. 274: 22114—22121. PMID 10419541.
- ^ Zhang, X., Meining, W., Fischer, M., Bacher, A. and Ladenstein, R. (2001). „X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons”. J. Mol. Biol. 306: 1099—1114. PMID 11237620.
- ^ Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J.P., Huber, R. and Bacher, A. (2002). „Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe”. Eur. J. Biochem. 269: 519—526. PMID 11856310.
- ^ Cushman, M., Yang, D., Gerhardt, S., Huber, R., Fischer, M., Kis, K. and Bacher, A. (2002). „Design, synthesis, and evaluation of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase”. J. Org. Chem. 67: 5807—5816. PMID 12153285.
- ^ Haase, I., Mortl, S., Kohler, P., Bacher, A. and Fischer, M. (2003). „Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii”. Eur. J. Biochem. 270: 1025—1032. PMID 12603336.
- ^ Morgunova, E., Meining, W., Illarionov, B., Haase, I., Jin, G., Bacher, A., Cushman, M., Fischer, M. and Ladenstein, R. (2005). „Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors”. Biochemistry. 44: 2746—2758. PMID 15723519.
- ^ Morgunova, E., Saller, S., Haase, I., Cushman, M., Bacher, A., Fischer, M. and Ladenstein, R. (2007). „Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design”. J. Biol. Chem. 282: 17231—17241. PMID 17446177.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze уреди
- 6,7-dimethyl-8-ribityllumazine+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
