Agaraza
Agaraza (EC 3.2.1.81, AgaA, AgaB, endo-beta-agaraza, agarozna 3-glikanohidrolaza) je enzim sa sistematskim imenom agaroza 4-glikanohidrolaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Agaraza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.81 | ||||||||
| CAS broj | 37288-57-6 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- hidroliza (1->4)-beta-D-galaktozidinih veza u agarozi, kojom se formira tetramer kao predominantni produkt
Ovaj enzim takođe deluje on porfiran.
Reference уреди
- ^ Duckworth, M. & Turvey, J.R. (1969). „The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran”. Biochem. J. 113: 687—692. PMID 5386190.
- ^ Allouch, J., Jam, M., Helbert, W., Barbeyron, T., Kloareg, B., Henrissat, B. and Czjzek, M. (2003). „The three-dimensional structures of two β-agarases”. J. Biol. Chem. 278: 47171—47180. PMID 12970344.
- ^ Ohta, Y., Nogi, Y., Miyazaki, M., Li, Z., Hatada, Y., Ito, S. and Horikoshi, K. (2004). „Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from the novel marine isolate, JAMB-A94”. Biosci. Biotechnol. Biochem. 68: 1073—1081. PMID 15170112.
- ^ Ohta, Y., Hatada, Y., Nogi, Y., Miyazaki, M., Li, Z., Akita, M., Hidaka, Y., Goda, S., Ito, S. and Horikoshi, K. (2004). „Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from a novel species of deep-sea Microbulbifer”. Appl. Microbiol. Biotechnol. 64: 505—514. PMID 15088129.
- ^ Sugano, Y., Terada, I., Arita, M., Noma, M. and Matsumoto, T. (1993). „Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107”. Appl. Environ. Microbiol. 59: 1549—1554. PMID 8517750.
- ^ Jam, M., Flament, D., Allouch, J., Potin, P., Thion, L., Kloareg, B., Czjzek, M., Helbert, W., Michel, G. and Barbeyron, T. (2005). „The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours”. Biochem. J. 385: 703—713. PMID 15456406.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze уреди
- Beta-agarase на US National Library of Medicine Medical Subject Headings (MeSH)
