Asparaginska sintaza (glutamin-hidrolizujuća)
Asparaginska sintaza (glutamin-hidrolizujuća) (EC 6.3.5.4, asparaginska sintetaza (glutaminska hidroliza), glutamin-zavisna asparaginska sintetaza, asparaginska sintetaza B, AS, AS-B) je enzim sa sistematskim imenom L-aspartat:L-glutamin amido-ligaza (formira AMP).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Asparaginska sintaza (glutamin-hidrolizujuća) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Asparaginska sintetaza B, dimer, E.Coli | |||||||||
| Identifikatori | |||||||||
| EC broj | 6.3.5.4 | ||||||||
| CAS broj | 37318-72-2 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- ATP + L-aspartat + L-glutamin + H2O AMP + difosfat + L-asparagin + L-glutamat (sveukupna reakcija)
- (1a) L-glutamin + H2O L-glutamat + NH3
- (1b) ATP + L-aspartat + NH3 AMP + difosfat + L-asparagin
Enzim iz Escherichia coli ima dva aktivna mesta.
Reference
уреди- ^ Patterson, M.K., Jr. & Orr, G.R. (1968). „Asparagine biosynthesis by the Novikoff hepatoma. Isolation, purification, property, and mechanism studies of the enzyme system”. J. Biol. Chem. 243: 376—380. PMID 4295091.
- ^ Boehlein, S.K., Richards, N.G. and Schuster, S.M. (1994). „Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad”. J. Biol. Chem. 269: 7450—7457. PMID 7907328.
- ^ Richards, N.G. & Schuster, S.M. (1998). „Mechanistic issues in asparagine synthetase catalysis”. Adv. Enzymol. Relat. Areas Mol. Biol. 72: 145—198. PMID 9559053.
- ^ Larsen, T.M., Boehlein, S.K., Schuster, S.M., Richards, N.G., Thoden, J.B., Holden, H.M. and Rayment, I. (1999). „Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product”. Biochemistry. 38: 16146—16157. PMID 10587437.
- ^ Huang, X., Holden, H.M. and Raushel, F.M. (2001). „Channeling of substrates and intermediates in enzyme-catalyzed reactions”. Annu. Rev. Biochem. 70: 149—180. PMID 11395405.
- ^ Tesson, A.R., Soper, T.S., Ciustea, M. and Richards, N.G. (2003). „Revisiting the steady state kinetic mechanism of glutamine-dependent asparagine synthetase from Escherichia coli”. Arch. Biochem. Biophys. 413: 23—31. PMID 12706338.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- Asparagine+synthase+(glutamine-hydrolysing) на US National Library of Medicine Medical Subject Headings (MeSH)
