Beta-karoten 3-hidroksilaza
Beta-karoten 3-hidroksilaza (EC 1.14.13.129, beta-karotenska 3,3'-monooksigenaza, CrtZ) je enzim sa sistematskim imenom beta-karoten,NADH:kiseonik 3-oksidoreduktaza.[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Beta-karoten 3-hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.129 | ||||||||
| CAS broj | 133425-64-6 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- beta-karoten + 2 NADH + 2 H+ + 2 O2 zeaksantin + 2 NAD+ + 2H2O
(sveukupna reakcija)
- (1a) beta-karoten + NADH + H+ + O2 beta-kriptoksantin + NAD+ + H2O
- (1b) beta-kriptoksantin + NADH + H+ + O2 zeaksantin + NAD+ + H2O
Za rado ovog enzima je neophodan feredoksin i Fe(II). On takođe deluje on druge karotenoide sa beta-krajnjom grupom.
Reference
уреди- ^ Sun, Z., Gantt, E. and Cunningham, F.X., Jr. (1996). „Cloning and functional analysis of the β-carotene hydroxylase of Arabidopsis thaliana”. J. Biol. Chem. 271: 24349—24352. PMID 8798688.
- ^ Fraser, P.D., Miura, Y. and Misawa, N. (1997). „In vitro characterization of astaxanthin biosynthetic enzymes”. J. Biol. Chem. 272: 6128—6135. PMID 9045623.
- ^ Fraser, P.D., Shimada, H. and Misawa, N. (1998). „Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay”. Eur. J. Biochem. 252: 229—236. PMID 9523693.
- ^ Bouvier, F., Keller, Y., d'Harlingue, A. and Camara, B. (1998). „Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.)”. Biochim. Biophys. Acta. 1391: 320—328. PMID 9555077.
- ^ Linden, H. (1999). „Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation”. Biochim. Biophys. Acta. 1446: 203—212. PMID 10524195.
- ^ Zhu, C., Yamamura, S., Nishihara, M., Koiwa, H. and Sandmann, G. (2003). „cDNAs for the synthesis of cyclic carotenoids in petals of Gentiana lutea and their regulation during flower development”. Biochim. Biophys. Acta. 1625: 305—308. PMID 12591618.
- ^ Choi, S.K., Matsuda, S., Hoshino, T., Peng, X. and Misawa, N. (2006). „Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli”. Appl. Microbiol. Biotechnol. 72: 1238—1246. PMID 16614859.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- Beta-carotene+3-hydroxylase на US National Library of Medicine Medical Subject Headings (MeSH)
