Cijanohidrin b-glukoziltransferaza
Cijanohidrin b-glukoziltransferaza (EC 2.4.1.85, uridin difosfoglukoza-p-hidroksimandelonitrilna glukoziltransferaza, UDP-glukoza-p-hidroksimandelonitrilna glukoziltransferaza, uridin difosfoglukoza-cijanohidrinska glukoziltransferaza, uridin difosfoglukoza:aldehid cijanohidrin beta-glukoziltransferaza, UDP-glukoza:(S)-4-hidroksimandelonitrilna beta-D-glukoziltransferaza, UGT85B1, UDP-glukoza:p-hidroksimandelonitril-O-glukoziltransferaza) je enzim sa sistematskim imenom UDP-D-glukoza:(S)-4-hidroksimandelonitril beta-D-glukoziltransferaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Cijanohidrin b-glukoziltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.4.1.85 | ||||||||
| CAS broj | 55354-52-4 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- UDP-D-glukoza + (S)-4-hidroksimandelonitril UDP + (S)-4-hidroksimandelonitril beta-D-glukozid
Ovaj enzim deluje na širok opseg supstrata in vitro, uključujući cijanohidrine, terpenoide, fenolne, heksanolne derivate i biljne hormone.
Reference уреди
- ^ Reay, P.F. & Conn, E.E. (1974). „The purification and properties of a uridine diphosphate glucose: aldehyde cyanohydrin β-glucosyltransferase from sorghum seedlings”. J. Biol. Chem. 249: 5826—5830. PMID 4416442.
- ^ Jones, P.R., Møller, B.L. and Hoj, P.B. (1999). „The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity”. J. Biol. Chem. 274: 35483—35491. PMID 10585420.
- ^ Hansen, K.S., Kristensen, C., Tattersall, D.B., Jones, P.R., Olsen, C.E., Bak, S. and Møller, B.L. (2003). „The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor”. Phytochemistry. 64: 143—151. PMID 12946413.
- ^ Busk, P.K. and Møller, B.L. (2002). „Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants”. Plant Physiol. 129: 1222—1231. PMID 12114576.
- ^ Kristensen, C., Morant, M., Olsen, C.E., Ekstrøm, C.T., Galbraith, D.W., Møller, B.L. and Bak, S. (2005). „Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome”. Proc. Natl. Acad. Sci. USA. 102: 1779—1784. PMID 15665094.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze уреди
- Cyanohydrin+beta-glucosyltransferase на US National Library of Medicine Medical Subject Headings (MeSH)
