D-Ala-D-Ala dipeptidaza (EC 3.4.13.22, D-alanil-D-alanin dipeptidaza, vanX D-Ala-D-Ala dipeptidaza, VanX) je enzim.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

D-Ala-D-Ala dipeptidaza
Identifikatori
EC broj3.4.13.22
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum
D-Ala-D-Ala + H2O 2 D-Ala

Ovaj enzim je zavistan od Zn2+ jona.

Reference

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  1. ^ Reynolds, P.E., Depardieu, F., Dutka-Malen, S., Arthur, M. and Courvalin, P. (1994). „Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine”. Mol. Microbiol. 13: 1065—1070. PMID 7854121. 
  2. ^ Wu, Z., Wright, G.D. and Walsh, C.T. (1995). „Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147”. Biochemistry. 34: 2455—2463. PMID 7873524. 
  3. ^ McCafferty, D.G., Lessard, I.A. and Walsh, C.T. (1997). „Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX”. Biochemistry. 36: 10498—10505. PMID 9265630. 
  4. ^ Bussiere, D.E., Pratt, S.D., Katz, L., Severin, J.M., Holzman, T. and Park, C.H. (1998). „The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance”. Mol. Cell. 2: 75—84. PMID 9702193. 
  5. ^ Tan, A.L., Loke, P. and Sim, T.S. (2002). „Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2)”. Res. Microbiol. 153: 27—32. PMID 11881895. 
  6. ^ Matthews, M.L., Periyannan, G., Hajdin, C., Sidgel, T.K., Bennett, B. and Crowder, M.W. (2006). „Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-substituted enzyme”. J. Am. Chem. Soc. 128: 13050—13051. PMID 17017774. 

Literatura

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Spoljašnje veze

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