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DNK polimeraza beta (POLB) je enzim koji kod ljudi kodira POLB gen.[1]

DNK polimeraza beta
PDB prikaz baziran na 1bno.
Dostupne strukture
1BPX, 1BPY, 1BPZ, 1MQ2, 1MQ3, 1TV9, 1TVA, 1ZJM, 1ZJN, 1ZQA, 1ZQB, 1ZQC, 1ZQD, 1ZQE, 1ZQF, 1ZQG, 1ZQH, 1ZQI, 1ZQJ, 1ZQK, 1ZQL, 1ZQM, 1ZQN, 1ZQO, 1ZQP, 1ZQQ, 1ZQR, 1ZQS, 1ZQT, 2FMP, 2FMQ, 2FMS, 2I9G, 2ISO, 2ISP, 2P66, 2PXI, 3C2K, 3C2L, 3C2M, 3GDX, 3ISB, 3ISC, 3ISD, 3JPN, 3JPO, 3JPP, 3JPQ, 3JPR, 3JPS, 3JPT, 3LK9, 3MBY, 3OGU, 3RH4, 3RH5, 3RH6, 3RJE, 3RJF, 3RJG, 3RJH, 3RJI, 3RJJ, 3RJK, 3TFR, 3TFS, 7ICE, 7ICF, 7ICG, 7ICH, 7ICI, 7ICJ, 7ICK, 7ICL, 7ICM, 7ICN, 7ICO, 7ICP, 7ICQ, 7ICR, 7ICS, 7ICT, 7ICU, 7ICV, 8ICA, 8ICB, 8ICC, 8ICE, 8ICF, 8ICG, 8ICH, 8ICI, 8ICJ, 8ICK, 8ICL, 8ICM, 8ICN, 8ICO, 8ICP, 8ICQ, 8ICR, 8ICS, 8ICT, 8ICU, 8ICV, 8ICW, 8ICX, 8ICY, 8ICZ, 9ICA, 9ICB, 9ICC, 9ICE, 9ICF, 9ICG, 9ICH, 9ICI, 9ICJ, 9ICK, 9ICL, 9ICM, 9ICN, 9ICO, 9ICP, 9ICQ, 9ICR, 9ICS, 9ICT, 9ICU, 9ICV, 9ICW, 9ICX, 9ICY
Identifikatori
Simboli POLB; MGC125976
Vanjski ID OMIM174760 MGI97740 HomoloGene2013 GeneCards: POLB Gene
EC broj 2.7.7.7
Pregled RNK izražavanja
PBB GE POLB 203616 at tn.png
podaci
Ortolozi
Vrsta Čovek Miš
Entrez 5423 18970
Ensembl ENSG00000070501 ENSMUSG00000031536
UniProt P06746 Q8K409
RefSeq (mRNA) NM_002690.2 NM_011130.2
RefSeq (protein) NP_002681.1 NP_035260.1
Lokacija (UCSC) Chr 8:
42.2 - 42.23 Mb
Chr 8:
23.74 - 23.76 Mb
PubMed pretraga [1] [2]
Regulatorni element u POLB
RF01455.png
Predviđena sekundarna struktura stem loopII (M2) regulatornog elementa POLB
Identifikatori
Simbol POLB
Rfam RF01455
Entrez 5423
HUGO POLB
OMIM 174760
RefSeq NM_002690
Drugi podaci
RNK tip Cis-reg
Domain(i) Mammalia
Lokus Hromozom 8 p11.2

FunkcijaУреди

U eukariotskim ćelijama, DNK polimeraza beta (POLB) izvodi popravke ekscizijom baza (BER) koje su neophodne za popravku DNK, replikaciju, rekombinaciju, i otpornost na lekove.[1]

Regulacija ekspresijeУреди

DNK polimeraza beta održava genomski integritet putem uzimanja učešća u popravci ekscizijom baza. Prekomerno izražavanje POLB iRNK je u korelaciji sa brojnim tipovima kancera, dok deficijencije POLB-a rezultuju u hipersenzitivnosti na alkilirajuće agense, uključujući apoptozu, i prekid hromozoma.[2] Iz tih razloga, esencijalno je precizno održavanje kontrole POLB ekspresije.[3][4][5][6]

InterakcijeУреди

DNA polimeraza beta formira interakcije sa PNKP[7] i XRCC1.[8][9][10][11]

ReferenceУреди

  1. 1,0 1,1 „Entrez Gene: POLB polymerase (DNA directed), beta”. 
  2. ^ Narayan S, He F, Wilson SH (1996). „Activation of the human DNA polymerase beta promoter by a DNA-alkylating agent through induced phosphorylation of cAMP response element-binding protein-1”. J. Biol. Chem. 271 (31): 18508—13. PMID 8702497. doi:10.1074/jbc.271.31.18508. 
  3. ^ Y, Canitrot; C, Cazaux; Fréchet M; et al. (1998). „Overexpression of DNA polymerase beta in cell results in a mutator phenotype and a decreased sensitivity to anticancer drugs”. Proc. Natl. Acad. Sci. U.S.A. 95 (21): 12586—90. PMC 22874 . PMID 9770529. doi:10.1073/pnas.95.21.12586. 
  4. ^ V, Bergoglio; MJ, Pillaire; Lacroix-Triki M; et al. (2002). „Deregulated DNA polymerase beta induces chromosome instability and tumorigenesis”. Cancer Res. 62 (12): 3511—4. PMID 12067997. 
  5. ^ V, Bergoglio; Y, Canitrot; Hogarth L; et al. (2001). „Enhanced expression and activity of DNA polymerase beta in human ovarian tumor cells: impact on sensitivity towards antitumor agents”. Oncogene. 20 (43): 6181—7. PMID 11593426. doi:10.1038/sj.onc.1204743. 
  6. ^ Srivastava DK, Husain I, Arteaga CL, Wilson SH (1999). „DNA polymerase beta expression differences in selected human tumors and cell lines”. Carcinogenesis. 20 (6): 1049—54. PMID 10357787. doi:10.1093/carcin/20.6.1049. 
  7. ^ Whitehouse, C J; Taylor R M; Thistlethwaite A; Zhang H; Karimi-Busheri F; Lasko D D, Weinfeld M; Caldecott K W (2001). „XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair”. Cell. United States. 104 (1): 107—17. ISSN 0092-8674. PMID 11163244. doi:10.1016/S0092-8674(01)00195-7. 
  8. ^ Wang, Liming; Bhattacharyya Nandan; Chelsea Diane M; Escobar Pedro F, Banerjee Sipra (2004). „A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype”. Cancer Res. United States. 64 (21): 7673—7. ISSN 0008-5472. PMID 15520167. doi:10.1158/0008-5472.CAN-04-2801. 
  9. ^ Fan, Jinshui; Otterlei Marit (2004). „XRCC1 co-localizes and physically interacts with PCNA”. Nucleic Acids Res. England. 32 (7): 2193—201. PMC 407833 . PMID 15107487. doi:10.1093/nar/gkh556. 
  10. ^ Kubota, Y; Nash R A (1996). „Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein”. EMBO J. ENGLAND. 15 (23): 6662—70. ISSN 0261-4189. PMC 452490 . PMID 8978692. 
  11. ^ Bhattacharyya, N; Banerjee S (2001). „A novel role of XRCC1 in the functions of a DNA polymerase beta variant”. Biochemistry. United States. 40 (30): 9005—13. ISSN 0006-2960. PMID 11467963. doi:10.1021/bi0028789. 

LiteraturaУреди

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