Glutamat sintaza (NADPH)
Glutamat sintaza (NADPH) (EC 1.4.1.13, glutamat (redukovani nikotinamid adenin dinukleotid fosfat) sintaza, L-glutamat sintaza, L-glutamat sintetaza, glutamatna sintetaza (NADP), NADPH-zavisna glutamatna sintaza, glutamin-ketoglutarinska aminotransferaza, NADPH-glutamatna sintaza, NADPH-vezana glutamatna sintaza, glutamin amid-2-oksoglutaratna aminotransferaza (oksidoreduktaza, NADP), L-glutamin:2-oksoglutarat aminotransferaza, NADPH oksidacija, GOGAT) je enzim sa sistematskim imenom L-glutamat:NADP+ oksidoreduktaza (transaminacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Glutamat sintaza (NADPH) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Glutamate synthase dodekamer, Azospirillum br. | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.4.1.13 | ||||||||
| CAS broj | 37213-53-9 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2 L-glutamat + NADP+ L-glutamin + 2-oksoglutarat + NADPH + H+ (sveukupna reakcija)
- (1a) L-glutamat + NH3 L-glutamin +H2O
- (1b) L-glutamat + NADP+ +H2O NH3 + 2-oksoglutarat + NADPH + H+
Ovaj enzim vezuje FMN, FAD, dva 4Fe-4S klustera i jedan 3Fe-4S klustera. Reakcija teče u reverznom smeru. Protein se sastoji od dve podjedinice, alfa and beta.
Reference уреди
- ^ Miller, R.E. & Stadtman, E.R. (1972). „Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein”. J. Biol. Chem. 247: 7407—7419. PMID 4565085.
- ^ Tempest, D.W., Meers, J.L. and Brown, C.M. (1970). „Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route”. Biochem. J. 117: 405—407. PMID 5420057.
- ^ Vanoni, M.A. & Curti, B. (1999). „Glutamate synthase: a complex iron-sulfur flavoprotein”. Cell. Mol. Life Sci. 55: 617—638. PMID 10357231.
- ^ Ravasio, S., Curti, B. and Vanoni, M.A. (2001). „Determination of the midpoint potential of the FAD and FMN flavin cofactors and of the 3Fe-4S cluster of glutamate synthase”. Biochemistry. 40: 5533—5541. PMID 11331018.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze уреди
- Glutamate+synthase+(NADPH) на US National Library of Medicine Medical Subject Headings (MeSH)
