Klavaminat sintaza
Klavaminat sintaza (EC 1.14.11.21, klavaminatna sintaza 2, sintaza klavaminske kiseline) je enzim sa sistematskim imenom dezoksiamidinoproklavaminat,2-oksoglutarat:kiseonik oksidoreduktaza (3-hidroksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Klavaminat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.11.21 | ||||||||
| CAS broj | 122799-56-8 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) dezoksiamidinoproklavaminat + 2-oksoglutarat + O2 amidinoproklavaminat + sukcinat + CO2
- (2) proklavaminat + 2-oksoglutarat + O2 dihidroklavaminat + sukcinat + CO2 +H2O
- (3) dihidroklavaminat + 2-oksoglutarat + O2 klavaminat + sukcinat + CO2 +H2O
Ovaj enzim sadrži gvožđe nevezano za hem. On katalizuje tri zasebne oksidativne reakcije u biositezi beta-laktamaznog inhibitora klavulanata kod Streptomyces clavuligerus.
Reference
уреди- ^ Salowe, S.P., Krol, W.J., Iwatareuyl, D. and Townsend, C.A. (1991). „Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction”. Biochemistry. 30: 2281—2292. PMID 1998687.
- ^ Zhou, J., Gunsior, M., Bachmann, B.O., Townsend, C.A. and Solomon, E.I. (1998). „Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation”. J. Am. Chem. Soc. 120: 13539—13540.
- ^ Zhang, Z.H., Ren, J.S., Stammers, D.K., Baldwin, J.E., Harlos, K. and Schofield, C.J. (2000). „Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase”. Nat. Struct. Biol. 7: 127—133. PMID 10655615.
- ^ Zhou, J., Kelly, W.L., Bachmann, B.O., Gunsior, M., Townsend, C.A. and Solomon, E.I. (2001). „Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional α-KG-dependent non-heme iron enzyme: Correlation with mechanisms and reactivities”. J. Am. Chem. Soc. 123: 7388—7398. PMID 11472170.
- ^ Townsend, C.A. (2002). „New reactions in clavulanic acid biosynthesis”. Curr. Opin. Chem. Biol. 6: 583—589. PMID 12413541.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- Clavaminate+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
