PreQ1 sintaza (EC 1.7.1.13, YkvM, QueF, preQ0 reduktaza, preQ0 oksidoreduktaza, 7-cijano-7-deazaguaninska reduktaza) je enzim sa sistematskim imenom 7-aminometil-7-karbaguanin:NADP+ oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

PreQ1 sintaza
Identifikatori
EC broj1.7.1.13
CAS broj1256460-80-6
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum
7-aminometil-7-karbaguanin + 2 NADP+ 7-cijano-7-karbaguanin + 2 NADPH + 2 H+

Reakcija se odvija u revernom smeru. Ovaj enzim katalizuje jedan od ranih koraka sinteze kjuozina (Q-tRNK). Njemu sledi reakcija posredovana enzimom EC 2.4.2.29, kjuozin tRNK-riboziltransferaze.

Reference

уреди
  1. ^ Van Lanen, S.G., Reader, J.S., Swairjo, M.A., de Crécy-Lagard, V., Lee, B. and Iwata-Reuyl, D. (2005). „From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold”. Proc. Natl. Acad. Sci. USA. 102: 4264—4269. PMID 15767583. 
  2. ^ Yokoyama, S., Miyazawa, T., Iitaka, Y., Yamaizumi, Z., Kasai, H. and Nishimura, S. (1979). „Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA”. Nature. 282: 107—109. PMID 388227. 
  3. ^ Kuchino, Y., Kasai, H., Nihei, K. and Nishimura, S. (1976). „Biosynthesis of the modified nucleoside Q in transfer RNA”. Nucleic Acids Res. 3: 393—398. PMID 1257053. 
  4. ^ Okada, N., Noguchi, S., Nishimura, S., Ohgi, T., Goto, T., Crain, P.F. and McCloskey, J.A. (1978). „Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine”. Nucleic Acids Res. 5: 2289—2296. PMID 353740. 
  5. ^ Noguchi, S., Yamaizumi, Z., Ohgi, T., Goto, T., Nishimura, Y., Hirota, Y. and Nishimura, S. (1978). „Isolation of Q nucleoside precursor present in tRNA of an E. coli mutant and its characterization as 7-(cyano)-7-deazaguanosine”. Nucleic Acids Res. 5: 4215—4223. PMID 364423. 
  6. ^ Swairjo, M.A., Reddy, R.R., Lee, B., Van Lanen, S.G., Brown, S., de Crécy-Lagard, V., Iwata-Reuyl, D. and Schimmel, P. (2005). „Crystallization and preliminary X-ray characterization of the nitrile reductase QueF: a queuosine-biosynthesis enzyme”. Acta Crystallogr. F Struct. Biol. Cryst. Commun. 61: 945—948. PMID 16511203. 

Literatura

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Spoljašnje veze

уреди