Di-trans,poli-cis-dekaprenilcistransferaza
(преусмерено са Sintetaza)
Di-trans,poli-cis-dekaprenilcistransferaza (EC 2.5.1.31, di-trans,poli-cis-undekaprenil-difosfat sintaza, undekaprenil-difosfat sintaza, baktoprenil-difosfatna sintaza, UPP sintetaza, undekaprenil difosfatna sintetaza, undekaprenilna pirofosfat, sintetaza) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:izopentenil-difosfat cistransferaza (dodaje 8 izopentenilnih jedinica).[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Di-trans,poli-cis-dekaprenilcistransferaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 2.5.1.31 | ||||||||
| CAS broj | 52350-87-5 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- (2E,6E)-farnezil difosfat + 8 izopentenil difosfat 8 difosfat + ditrans,oktacis-undekaprenil difosfat
Undekaprenil pirofosfatna sintaza katalizuje konsekutivne reakcije kondenzacije farnezil difosfata sa osam izopentenilnih difosfata, u kojima se nove cis-dvostruke veze formiraju. Time se generiše undekaprenil difosfat koji služi kao lipidni nosilac za peptidoglikansku sintezu bakterijskog ćelijskog zida.
Reference
уреди- ^ Muth, J.D. & Allen, C.M. (1984). „Undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum: a dimeric protein”. Arch. Biochem. Biophys. 230: 49—60. PMID 6712246.
- ^ Takahashi, I. & Ogura, K. (1982). „Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase”. J. Biochem. (Tokyo). 92: 1527—1537. PMID 6818223.
- ^ Guo, R.T., Ko, T.P., Chen, A.P., Kuo, C.J., Wang, A.H. and Liang, P.H. (2005). „Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis”. J. Biol. Chem. 280: 20762—20774. PMID 15788389.
- ^ Ko, T.P., Chen, Y.K., Robinson, H., Tsai, P.C., Gao, Y.G., Chen, A.P., Wang, A.H. and Liang, P.H. (2001). „Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis”. J. Biol. Chem. 276: 47474—47482. PMID 11581264.
- ^ Fujikura, K., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. Biochemistry. 42: 4035—4041. PMID 12680756.
- ^ Fujihashi, M., Zhang, Y.W., Higuchi, Y., Li, X.Y., Koyama, T. and Miki, K. (2001). „Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase”. Proc. Natl. Acad. Sci. USA. 98: 4337—4342. PMID 11287651.
- ^ Pan, J.J., Chiou, S.T. and Liang, P.H. (2000). „Product distribution and pre-steady-state kinetic analysis of Escherichia coli undecaprenyl pyrophosphate synthase reaction”. Biochemistry. 39: 10936—10942. PMID 10978182.
- ^ Kharel, Y., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. J. Biochem. 134: 819—826. PMID 14769870.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- Ditrans,polycis-undecaprenyl-diphosphate+synthase+((2E,6E)-farnesyl-diphosphate+specific) на US National Library of Medicine Medical Subject Headings (MeSH)
