Tiamin oksidaza
Tiamin oksidaza (EC 1.1.3.23, tiaminska dehidrogenaza, tiaminska dehidrogenaza, tiamin:kiseonik 5-oksidoreduktaza) je enzim sa sistematskim imenom tiamin:kiseonik 5-oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Tiamin oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.3.23 | ||||||||
| CAS broj | 96779-44-1 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- tiamin + 2 O2 +H2O tiamin sirćetna kiselina + 2H2O2
Ovaj enzim je flavoprotein (FAD). Njegov produkt se razlikuje od tiamin po zameni grupe -CH2.CH2.OH grupom -CH2.COOH. Dvostepena oksidacija teče bez otpuštanja intermedijernog aldehida iz enzima.
Reference
уреди- ^ Edmondson, D.E., Kenney, W.C. and Singer, T.P. (1976). „Structural elucidation and properties of 8α-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and β-cyclopiazonate oxidocyclase”. Biochemistry. 15: 2937—2945. PMID 8076.
- ^ Gomez-Moreno, C. & Edmondson, D.E. (1985). „Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase”. Arch. Biochem. Biophys. 239: 46—52. PMID 2988447.
- ^ Neal, R.A. (1970). „Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2′-methyl-4′-amino-5′-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism”. J. Biol. Chem. 245: 2599—2604. PMID 4987737.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- Thiamine+oxidase на US National Library of Medicine Medical Subject Headings (MeSH)
