Versatil peroksidaza
Versatil peroksidaza (EC 1.11.1.16, VP, hibridna peroksidaza, polivalentna peroksidaza) je enzim sa sistematskim imenom reaktivno-crno-5:vodonik-peroksid oksidoreduktaza.[1][2][3][4][5][6][7][8][9][10] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Versatil peroksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.11.1.16 | ||||||||
| CAS broj | 114995-15-2&title= 42613-30-9, 114995-15-2 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) boja reaktivno crno 5 + H2O2 boja oksidovano reaktivno crno 5 + 2H2O
- (2) donor + H2O2 oksidovani donor + 2H2O
Ovaj enzim je hemoprotein. Ligninolitička peroksidaza kombinuje supstratno-specifične karakteristike dve druge ligninolitičke peroksidaze, EC 1.11.1.13, mangan peroksidaze i EC 1.11.1.14, lignin peroksidaze. On takođe može da oksiduje fenole, hidrohinone i boje.
Reference
уреди- ^ Martínez, M.J., Ruiz-Dueñas, F.J., Guillén, F. and Martínez, A.T. (1996). „Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii”. Eur. J. Biochem. 237: 424—432. PMID 8647081.
- ^ Heinfling, A., Ruiz-Dueñas, F.J., Martínez, M.J., Bergbauer, M., Szewzyk, U. and Martínez, A.T. (1998). „A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta”. FEBS Lett. 428: 141—146. PMID 9654123.
- ^ Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T. (1999). „Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii”. Mol. Microbiol. 31: 223—235. PMID 9987124.
- ^ Camarero, S., Sarkar, S., Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T. (1999). „Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites”. J. Biol. Chem. 274: 10324—10330. PMID 10187820.
- ^ Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T. (1999). „Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn2+ and different aromatic substrates”. Appl. Environ. Microbiol. 65: 4705—4707. PMID 10508113.
- ^ Camarero, S., Ruiz-Dueñas, F.J., Sarkar, S., Martínez, M.J. and Martínez, A.T. (2000). „The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases”. FEMS Microbiol. Lett. 191: 37—43. PMID 11004397.
- ^ Ruiz-Dueñas, F.J., Camarero, S., Pérez-Boada, M., Martínez, M.J. and Martínez, A.T. (2001). „A new versatile peroxidase from Pleurotus”. Biochem. Soc. Trans. 29: 116—122. PMID 11356138.
- ^ Banci, L., Camarero, S., Martínez, A.T., Martínez, M.J., Pérez-Boada, M., Pierattelli, R. and Ruiz-Dueñas, F.J. (2003). „NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii”. J. Biol. Inorg. Chem. 8: 751—760. PMID 12884090.
- ^ Pérez-Boada, M., Ruiz-Dueñas, F.J., Pogni, R., Basosi, R., Choinowski, T., Martínez, M.J., Piontek, K. and Martínez, A.T. (2005). „Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways”. J. Mol. Biol. 354: 385—402. PMID 16246366.
- ^ Caramelo, L., Martínez, M.J. and Martínez, A.T. (1999). „A search for ligninolytic peroxidases in the fungus Pleurotus eryngii involving α-keto-γ-thiomethylbutyric acid and lignin model dimer”. Appl. Environ. Microbiol. 65: 916—922. PMID 10049842.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- Versatile+peroxidase на US National Library of Medicine Medical Subject Headings (MeSH)
