Бифлавиолин синтаза
Biflaviolin sintaza (EC 1.14.21.7, CYP158A2, CYP 158A2, citohrom P450 158A2) je enzim sa sistematskim imenom flaviolin,NADPH:kiseonik oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Biflaviolin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.21.7 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) 2 flaviolin + NADPH + H+ + O2 3,3'-biflaviolin + NADP+ + 2H2O
- (2) 2 flaviolin + NADPH + H+ + O2 3,8'-biflaviolin + NADP+ + 2H2O
Ovaj enzim je citohrom-P450 iz zemljišne bakterije Streptomyces coelicolor A3. On katalizuje fenolnu oksidaciju C-C spajanja, koja dovodi do polimerizacije flaviolina čime se formira biflaviolin ili triflaviolin bez inkorporacije kiseonika u produkt.
Reference
уреди- ^ Zhao, B., Guengerich, F.P., Bellamine, A., Lamb, D.C., Izumikawa, M., Lei, L., Podust, L.M., Sundaramoorthy, M., Kalaitzis, J.A., Reddy, L.M., Kelly, S.L., Moore, B.S., Stec, D., Voehler, M., Falck, J.R., Shimada, T. and Waterman, M.R. (2005). „Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2”. J. Biol. Chem. 280: 11599—11607. PMID 15659395.
- ^ Zhao, B., Guengerich, F.P., Voehler, M. and Waterman, M.R. (2005). „Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transfer”. J. Biol. Chem. 280: 42188—42197. PMID 16239228.
- ^ Zhao, B., Lamb, D.C., Lei, L., Kelly, S.L., Yuan, H., Hachey, D.L. and Waterman, M.R. (2007). „Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2”. Biochemistry. 46: 8725—8733. PMID 17614370.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- Biflaviolin+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
