Holin oksidaza (EC 1.1.3.17, holinska oksidaza) je enzim sa sistematskim imenom holin:kiseonik 1-oksidoreduktaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

Holin oksidaza
Holin oksidaza dimer, Arthrobacter globiformis
Identifikatori
EC broj1.1.3.17
CAS broj9028-67-5
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum
holin + 2 O2 +H2O betain + 2H2O2 (sveukupna reakcija)
(1a) holin + O2 betain aldehid +H2O2
(1b) betain aldehid + O2 +H2O betain +H2O2

Ovaj enzim je flavoprotein (FAD). Kod mnogih bakterija, biljki i životinja, osmoprotektant betain se sintetiše koristeći različite enzime koji katalizuju konverziju: (1) holina u betainski aldehid i (2) betain aldehida u betain.

Reference

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  1. ^ Ikuta, S., Imamura, S., Misaki, H. and Horiuti, Y. (1977). „Purification and characterization of choline oxidase from Arthrobacter globiformis”. J. Biochem. (Tokyo). 82: 1741—1749. PMID 599154. 
  2. ^ Rozwadowski, K.L., Khachatourians, G.G. and Selvaraj, G. (1991). „Choline oxidase, a catabolic enzyme in Arthrobacter pascens, facilitates adaptation to osmotic stress in Escherichia coli. J. Bacteriol. 173: 472—478. PMID 1987142. 
  3. ^ Rand, T., Halkier, T. and Hansen, O.C. (2003). „Structural characterization and mapping of the covalently linked FAD cofactor in choline oxidase from Arthrobacter globiformis”. Biochemistry. 42: 7188—7194. PMID 12795615. 
  4. ^ Gadda, G., Powell, N.L. and Menon, P. (2004). „The trimethylammonium headgroup of choline is a major determinant for substrate binding and specificity in choline oxidase”. Arch. Biochem. Biophys. 430: 264—273. PMID 15369826. 
  5. ^ Fan, F. & Gadda, G. (2005). „On the catalytic mechanism of choline oxidase”. J. Am. Chem. Soc. 127: 2067—2074. PMID 15713082. 
  6. ^ Waditee, R., Tanaka, Y., Aoki, K., Hibino, T., Jikuya, H., Takano, J., Takabe, T. and Takabe, T. (2003). „Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica”. J. Biol. Chem. 278: 4932—4942. PMID 12466265. 
  7. ^ Fan, F., Ghanem, M. and Gadda, G. (2004). „Cloning, sequence analysis, and purification of choline oxidase from Arthrobacter globiformis: a bacterial enzyme involved in osmotic stress tolerance”. Arch. Biochem. Biophys. 421: 149—158. PMID 14678796. 
  8. ^ Gadda, G. (2003). „Kinetic mechanism of choline oxidase from Arthrobacter globiformis”. Biochim. Biophys. Acta. 1646: 112—118. PMID 12637017. 

Literatura

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Spoljašnje veze

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