Taksan 13a-hidroksilaza
Taksan 13a-hidroksilaza (EC 1.14.13.77, taksanska 13a-hidroksilaza) je enzim sa sistematskim imenom taksa-4(20),11-dien-5alfa-ol,NADPH:kiseonik oksidoreduktaza (13alfa-hidroksilacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
Taksan 13a-hidroksilaza | |||||||||
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Identifikatori | |||||||||
EC broj | 1.14.13.77 | ||||||||
CAS broj | 399030-58-1 | ||||||||
Baze podataka | |||||||||
IntEnz | IntEnz pregled | ||||||||
BRENDA | BRENDA pristup | ||||||||
ExPASy | NiceZyme pregled | ||||||||
KEGG | KEGG pristup | ||||||||
MetaCyc | metabolički put | ||||||||
PRIAM | profil | ||||||||
Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- taksa-4(20),11-dien-5alfa-ol + NADPH + H+ + O2 taksa-4(20),11-dien-5alfa,13alfa-diol + NADP+ + H2O
Ovaj mikrozomalni citohrom-P450-zavisni enzim učestvuje u biosinteza diterpenoidnog antineoplastičnog leka taksola (paklitaksela).
Reference
uredi- ^ Wheeler, A.L., Long, R.M., Ketchum, R.E., Rithner, C.D., Williams, R.M. and Croteau, R. (2001). „Taxol biosynthesis: differential transformations of taxadien-5α-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells”. Arch. Biochem. Biophys. 390: 265—78. PMID 11396929.
- ^ Jennewein, S., Rithner, C.D., Williams, R.M. and Croteau, R.B. (2001). „Taxol biosynthesis: taxane 13 α-hydroxylase is a cytochrome P450-dependent monooxygenase”. Proc. Natl. Acad. Sci. USA. 98: 13595—13560. PMID 11707604.
Literatura
uredi- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
uredi- Taxane+13alpha-hydroxylase na US National Library of Medicine Medical Subject Headings (MeSH)