Eritronolid sintaza
(преусмерено са 6-dezoksieritronolidna B sintaza)
Eritronolid sintaza (EC 2.3.1.94, eritronolid kondenzujući enzim, malonil-KoA:propionil-KoA maloniltransferaza (ciklizacija), eritronolidna sintaza, malonil-KoA:propanoil-KoA maloniltransferaza (ciklizacija), dezoksieritronolidna B sintaza, 6-dezoksieritronolidna B sintaza, DEBS) je enzim sa sistematskim imenom propanoil-KoA:(2S)-metilmalonil-KoA maloniltransferaza (ciklizacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Eritronolid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.3.1.94 | ||||||||
| CAS broj | 87683-77-0 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- propanoil-KoA + 6 (2S)-metilmalonil-KoA + 6 NADPH + 6 H+ 6-dezoksieritronolid B + 7 KoA + 6 CO2 +H2O + 6 NADP+
Produkt, 6-dezoksieritronolid B, sadrži 14-člani laktonski prsten. On je intermedijer u biosintezi eritromicinskih antibiotika.
Reference уреди
- ^ Omura, S. & Nakagawa, A. (1981). „Biosynthesis of 16-membered macrolide antibiotics”. Antibiotics. 4: 175—192.
- ^ Roberts, G. & Leadley, P.F. (1984). „Use of [3H]tetrahydrocerulenin to assay condensing enzyme activity in Streptomyces erythreus”. Biochem. Soc. Trans. 12: 642—643.
- ^ Pfeifer, B.A., Admiraal, S.J., Gramajo, H., Cane, D.E. and Khosla, C. (2001). „Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli”. Science. 291: 1790—1792. PMID 11230695.
- ^ Tsai, S.C., Miercke, L.J., Krucinski, J., Gokhale, R., Chen, J.C., Foster, P.G., Cane, D.E., Khosla, C. and Stroud, R.M. (2001). „Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel”. Proc. Natl. Acad. Sci. USA. 98: 14808—14813. PMID 11752428.
- ^ Khosla, C., Tang, Y., Chen, A.Y., Schnarr, N.A. and Cane, D.E. (2007). „Structure and mechanism of the 6-deoxyerythronolide B synthase”. Annu. Rev. Biochem. 76: 195—221. PMID 17328673.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze уреди
- Erythronolide+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
