Izocitrat dehidrogenaza (NAD+)
Izocitrat dehidrogenaza (NAD+) (EC 1.1.1.41, beta-ketoglutarinska-izocitratna karboksilaza, izocitratna kiselina dehidrogenaza, NAD zavisna izocitratna dehidrogenaza, NAD izocitratna dehidrogenaza, NAD-vezana izocitratna dehidrogenaza, NAD-specifična izocitratna dehidrogenaza, NAD izocitratna dehidrogenaza, izocitratna dehidrogenaza (NAD), nikotinamid adenin dinukleotid izocitratna dehidrogenaza) je enzim sa sistematskim imenom izocitrat:NAD+ oksidoreduktaza (dekarboksilacija).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Izocitrat dehidrogenaza (NAD+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 isocitrate dehydrogenase heterodimer, Human | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.1.1.41 | ||||||||
| CAS broj | 9001-58-5 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- izocitrat + NAD+ 2-oksoglutarat + CO2 + NADH
Neophodni su Mn2+ ili Mg2+ za aktivnost. Za razliku od EC 1.1.1.42, izocitratna dehidrogenaza (NADP+), ne može da koristi oksalosukcinat kao supstrat. Kod eukariota, izocitratna dehidrogenaza postoji u dve forme: NAD+-vezani enzim koji je prisutan u mitohondrijama i ima alosterna svojstva, i nealosterni, NADP+-vezani enzim koji je prisutan u mitohondrijama i u citoplazmi.
Reference уреди
- ^ Hathaway, J.A. & Atkinson, D.E. (1963). „The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism”. J. Biol. Chem. 238: 2875—2881. PMID 14063317.
- ^ Kornberg, A. & Pricer, W.E. (1951). „Di- and triphosphopyridine nucleotide isocitric dehydrogenase in yeast”. J. Biol. Chem. 189: 123—136. PMID 14832224.
- ^ Plaut, G.W.E. (1963). „Isocitrate dehydrogenases”. Ур.: Boyer, P.D., Lardy, H.; Myrb; auml; ck, K. The Enzymes. 7 (2nd изд.). New York: Academic Press. стр. 105—126.
- ^ Plaut, G.W.E. & Sung, S.-C. (1954). „Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues”. J. Biol. Chem. 207: 305—314. PMID 13152105.
- ^ Ramakrishnan, C.V. & Martin, S.M. (1955). „Isocitric dehydrogenase in Aspergillus niger”. Arch. Biochem. Biophys. 55: 403—407.
- ^ Vickery, H.B. (1962). „A suggested new nomenclature for the isomers of isocitric acid”. J. Biol. Chem. 237: 1739—1741. PMID 13925783.
- ^ Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. (1995). „Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence”. FEMS Microbiol. Lett. 134: 85—90. PMID 8593959.
- ^ Kim, Y.O., Koh, H.J., Kim, S.H., Jo, S.H., Huh, J.W., Jeong, K.S., Lee, I.J., Song, B.J. and Huh, T.L. (1999). „Identification and functional characterization of a novel, tissue-specific NAD+-dependent isocitrate dehydrogenase β subunit isoform”. J. Biol. Chem. 274: 36866—36875. PMID 10601238.
- ^ Inoue, H., Tamura, T., Ehara, N., Nishito, A., Nakayama, Y., Maekawa, M., Imada, K., Tanaka, H. and Inagaki, K. (2002). „Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans”. FEMS Microbiol. Lett. 214: 127—132. PMID 12204383.
Literatura уреди
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze уреди
- Isocitrate+dehydrogenase+(NAD+) на US National Library of Medicine Medical Subject Headings (MeSH)
