Steroid 15beta-monooksigenaza (EC 1.14.15.8, citohrom P-450meg, citohrom P450meg, steroidna 15beta-hidroksilaza, CYP106A2, BmCYP106A2) je enzim sa sistematskim imenom progesteron,redukovani-feredoksin:kiseonik oksidoreduktaza (15beta-hidroksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

Steroid 15beta-monooksigenaza
Identifikatori
EC broj1.14.15.8
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum
progesteron + redukovani feredoksin + O2 15beta-hidroksiprogesteron + oksidovani feredoksin + H2O

Enzim iz Bacillus megaterium vrši hidroksilaciju raznih 3-okso-Delta4-steroida u poziciji 15beta.

Reference

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  1. ^ Berg, A., Ingelman-Sundberg, M. and Gustafsson, J.A. (1979). „Purification and characterization of cytochrome P-450meg”. J. Biol. Chem. 254: 5264—5271. PMID 109432. 
  2. ^ Berg, A., Gustafsson, J.A. and Ingelman-Sundberg, M. (1976). „Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium”. J. Biol. Chem. 251: 2831—2838. PMID 177422. 
  3. ^ Lisurek, M., Kang, M.J., Hartmann, R.W. and Bernhardt, R. (2004). „Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry”. Biochem. Biophys. Res. Commun. 319: 677—682. PMID 15178459. 
  4. ^ Goni, G., Zollner, A., Lisurek, M., Velazquez-Campoy, A., Pinto, S., Gomez-Moreno, C., Hannemann, F., Bernhardt, R. and Medina, M. (2009). „Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368”. Biochim. Biophys. Acta. 1794: 1635—1642. PMID 19635596. 
  5. ^ Lisurek, M., Simgen, B., Antes, I. and Bernhardt, R. (2008). „Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation”. Chembiochem. 9: 1439—1449. PMID 18481342. 

Literatura

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Spoljašnje veze

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